First United Methodist Church in Roanoke Rapids donated $20,000 to the ECU Health North Hospital Development Council.
The funds will be earmarked for cancer care.
ECU North said when it became apparent in May that First United Methodist Church had to close its doors, church officials wanted to leave a legacy in the community.
Marion Hudson and Bill White made the donation on behalf of the church.
Becky Newton, chairman of the ECU Health North Development Council accepted the donation.